Cdc13 phosphorylation
WebBackground The fission yeast Schizosaccharomyces pombe is widely-used as a model organism for the study of a broad range of eukaryotic cellular processes such as cell cycle, genome stability and cell morphology. Despite the availability of extensive WebOct 16, 1998 · The supercoiling activity of 13Scondensin was regulated by mitosis-specific phosphorylation. Immunodepletion, in vitro phosphorylation, and peptide-mapping …
Cdc13 phosphorylation
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WebPhosphorylation of Cdc13 by Cdk1 promotes the interaction between Cdc13 and Est1 and hence telomere elongation. Here, we show that Cdk1 also phosphorylates Stn1 at threonine 223 and serine 250 both in vitro and in vivo, and these phosphorylation events are essential for the stability of the CST complexes at the telomeres. WebApr 15, 2005 · B, quantification of Cdc13 phosphorylation. The experiments described in A were repeated three times with the indicated strains in the left graph and three times with the strains indicated in the right graph. Gel bands containing [32 P]Cdc13p were excised and counted. Anti-Cdc13p immunoprecipitates prepared in parallel from unlabeled strains ...
WebJun 29, 2024 · The tightly regulated timing of mitosis in S. pombe occurs via the reciprocal activities of Cdc25 and Wee1 on Cdc2 inhibitory phosphorylation. Wee1 negatively regulates Cdc2 by phosphorylation of tyrosine residue 15 (Tyr15), and this is counteracted by the phosphatase activity of Cdc25 [1,2,3].Cells must advance or delay mitosis under … WebOct 3, 2011 · Cdc13 is thought to be a Tel1/Mec1 target as both kinases can phosphorylate N-terminal fragments of Cdc13 in vitro. Moreover, simultaneous mutation of two of the …
WebOur data indicate that Cdc13p accumulates in the nucleolus in response to G 2 checkpoint delays, rather than in the cytoplasm, and that … WebOct 30, 2012 · In fact, even though it was proposed that Cdc13 phosphorylation by Mec1/Tel1 was essential for the interaction between Cdc13 and Est1 , the latest data indicate that the mutagenesis of every potential consensus phosphorylation site for Tel1 confers nearly wild-type telomeres length and does not affect Cdc13-Est1 interaction .
WebCdk1-dependent phosphorylation of Cdc13 is essential for efficient recruitment of the yeast telomerase complex to telomeres by favoring the interaction of Cdc13 with Est1 …
WebNov 12, 2014 · As was mentioned above, Tel1/Mec1mediated Cdc13 phosphorylation promotes the Cdc13-Est1 interaction [82, 83]. In turn, this effect is counteracted by the PP2A phosphatase and the Aurora kinase [83 le boat crawleyWebJan 1, 2015 · Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13 Genetics Dec 2010 Other authors. See publication. More activity by Bari ... how to drive a car project zomboidWebNov 15, 2006 · The phosphorylated GST-Cdc13(129–332) subunit was observed, while neither GST alone (data not shown) nor the GST-Cdc13(570–676) protein showed detectable phosphorylation by Mec1p or Tel1p. To confirm that Mec1 kinase activity was indeed required for Cdc13 phosphorylation, assays were conducted using extracts … le boat chertsey jobsWebIn this last situation, Cdc2-Tyr15 phosphorylation could easily be detected in these HU-blocked cells despite the absence of Cdc13, showing that Cdc2/cyclin complex(es) other than Cdc2/Cdc13 are ... le boat chertsey marinaWebIn ciliate, phosphorylation of telomere end binding protein TEBP-β, a homolog of human TPP1, has been shown to regulate cell cycle-dependent telomerase recruitment ( 23 ). In budding yeast, regulation of cell cycle-dependent telomerase recruitment by the telomeric protein Cdc13 requires its phosphorylation by cyclin-dependent kinase 1 (Cdk1 ... how to drive across countryWeb(E) The expression of cdc13+ in WT (JY333) and ecls∆ (JY333 ecl1∆ ecl2∆ ecl3∆) cells during sulfur depletion (n = 3). (F) Levels of Cdc13 and the Cdc2-Tyr15 phosphorylation under sulfur depletion (n = 3). (G) Cells were collected 1 day after transfer to sulfur-depleted medium, and levels of Cdc13 were le boat hausbooteWebJun 26, 2024 · Indeed, the transition from the Cdc13-Est1 interaction to the CST complex formation seems to be regulated in part by sequential phosphorylation of Cdc13 and Stn1 by Cdk1 [89,90] (Figure 3D). le boat horizon 3